Next Generation Sequencing
The high throughput, next generation sequencing offered by ARK Genomics, using the Illumina MiSeq, the Illumina HiSeq 2500 system and the Illumina HiSeq 2000 system, offers the simplest and fastest workflow for a broad range of high-throughput sequencing applications. Sample libraries are prepared and clonal clusters are generated on Illumina sequencing flow cells using the cluster generation system. By using Illumina's sequencing by synthesis technology, hundreds of millions of clusters are sequenced in parallel, generating several hundred Gb of sequence information in a long, paired end sequencing run.
ARK Genomics can perform whole-genome or de novo sequencing, resequencing comparing to a reference genome and are developing targeted resequencing, SNP discovery, and identification of copy number variations and chromosomal rearrangements. ARK Genomics have the ability to multiplex up to 12 libraries in one flow cell lane, reducing the cost of this service for small genomic libraries. The HiSeq 2000 system gives flexibility of insert size and different length (36bp-100bp reads as standard; we have pushed the machine up to 150bp reads), single or paired-end protocols, enabling the broadest range of genomic sequencing applications. We ask for 10ug of genomic DNA starting material for this protocol.
mRNA Sequencing (Transcriptome Analysis)
ARK Genomics can perform transcriptome analysis using mRNA sequencing on the HiSeq platform. We can characterise transcriptional activity and explore RNA architecture without prior information. Data collated can be used to annotate coding SNPs, identify regulatory RNAs, characterize splice junctions and determine transcript abundance. The Genome analyser IIx system gives the flexibility of different length (36bp-100bp reads), single or paired-end protocols, enabling us to tailor a protocol for specific project requirements. It also allows us to align RNA sequencing reads across splice junctions and map to reference genomes, identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. We ask for 5ug of total RNA starting material for this protocol.
The small RNA sequencing offered by ARK Genomics enables the discovery and profiling of microRNAs and other non-coding RNA on any organism without prior genome annotation. Using low RNA inputs (1.0ug total RNA or less), you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions. The use of Illumina's small RNA sequencing methods allows accurate detection and quantification of rare small RNA sequences.
We have library preperation protocols for SNP discovery, RNA-Seq, ChIP-Seq, MeDIP-Seq, Bisulfite sequencing, microRNA-Seq, Metagenomics and 16s community profiling. Please see the individual webpages listed for further information on our services or please get in touch.